Pratip Chattopadhyay, Staff Scientist at National Institutes of Health, presented a Forensic Flow Cytometry Tutorial at the CYTO2015 Conference.
Use standard calibration protocols or develop your own.
Calibrate intensity scales to absolute units
Performance test of PMTs and signal processing
Filter Setting Validation
Accurate measurements of Q and B
For cytometers fluorescence beads are primarily a source of light. Light is emitted from a bead as it passes through the laser beam in the focus of the cytometer’s flow cell. As the beads rapidly run through the flow cell, the light they emit is recognized by the detectors as short light pulses.
quantiFlash® gives you precise light pulses with unsurpassed long-term stability. By replacing bead-based light pulses with quantiFlash, you gain robustness and reproducibility in your calibration routines.