quantiflash APE LED pulser cytometry calibration main image.png
We just think it's time for a worthy alternative.
We have nothing against beads in cytometry.
Bead-less cytometer calibration
Routine detector performance optimization
Long-term performance tracking


quantiFlash® - Calibration Light Source for Cytometry

  quantiFlash® is an ideal tool to predict the best resolution and sensitivity of cytometers. Unlike calibration beads, quantiFlash® delivers consistent and uniform light pulses. Their intensity and duration is freely adjustable.


Perfect light pulses with almost no variation outperform bead-based calibration particles.


A precise LED pulser light source is the ideal tool for monitoring and calibration in flow cytometry.


Use standard calibration protocols or develop your own.

There are so many things to do with quantiFlash.




Calibrate intensity scales to absolute units

Performance test of PMTs and signal processing

Filter Setting Validation

Accurate measurements of Q and B



What makes quantiFlash®different?

  •  No beads required
  •  Headache-free perfect light pulses
  •  A single universal device
  •  Ideally suited for daily calibration routine


For the first time, ideal light pulses are used instead of beads

Performance Test of PMTs and Signal Processing

• Use the proven perfect linearity of quantiFlash® to check the performance of your PMTs and signal processing
• Precise adjustment of the delivered light intensity within up to 6 decades

Accurate Measurement of Q and B

• Calculate values for detection efficiency (Q) and background (B) in terms of statistical photoelectron units (Spe) or fluorescence intensity units, such as mean equivalent soluble fluorescence units (MESF) or an equivalent number of reference fluorophores (ERF)
• Accurately compare different cytometers or setups

No Limits

Leave the limitation of 4, 6, or 8 peaks behind with an unlimited number of peaks

Calibrate Intensity Scales to Absolute Units

• Ultra-stable light pulses allow for direct calibration of intensity channels to a standardized photonic scale
• Easy calculation of the corresponding number of detected statistical photoelectrons (Spe) for any intensity channel

quantiflash LED pulser cytometer BD FACS ARIA PMT Channels

Performance Tracking & Result Comparison

Performance tracking in long-term or inter-laboratory studies as well as comparability of instruments become available with the quantiFlash® LED pulser.

quantiFlash® enables user to determine the resolution and sensitivity (reflected by Q and B) for each detector so that instrument comparison as well as long-term experiments become more efficient.


From calibration beads to calibration light

For cytometers fluorescence beads are primarily a source of light. Light is emitted from a bead as it passes through the laser beam in the focus of the cytometer’s flow cell. As the beads rapidly run through the flow cell, the light they emit is recognized by the detectors as short light pulses.

quantiFlash® gives you precise light pulses with unsurpassed long-term stability. By replacing bead-based light pulses with quantiFlash, you gain robustness and reproducibility in your calibration routines.


 Two button control

 Unlimited Pulses

 Unlimited Pulse Shapes

 PC Desktop Control


quantiFlash: Making LED pulse based technology available to the general scientific community for the first time.

Pratip Chattopadhyay, Staff Scientist at National Institutes of Health, presented a Forensic Flow Cytometry Tutorial at the CYTO2015 Conference.

Comparison study of various flow cytometers using quantiFlash. Traditionally, flow cytometers are characterized (sensitivity, linearity, long time stability etc.) by fluorescent microspheres.

Calibration of flow cytometric analysis and characterization of background noise sources that may impact EV fluorescence or scatter detection.